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KMID : 0191120010160060756
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Journal of Korean Medical Science
2001 Volume.16 No. 6 p.756 ~ p.761
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Hypermethylation of Tumor-related Genes in Genitourinary Cancer Cell Lines
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Jung Wan Kim/Woon Bok Chung
Su Hyung Hong/Jin A Kim/Yoon Kyung Sohn/Bup Wan Kim/Jung Wan Kim
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Abstract
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Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines.
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